An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division.
Cancer occurs when a somatic cell which normally cannot divide undergoes mutations which cause de-regulation of the normal cell cycle controls leading to uncontrolled proliferation. Una linea cellulare immortale è una popolazione di cellule di un organismo multicellulare che normalmente non proliferano indefinitamente ma, a causa di mutazione, hanno evitato normale senescenza cellulare e invece può mantenere in fase di divisione. The addition of nerve growth factor (NGF) causes PC12 cells to reversibly differentiate into a neuronal phenotype (Addition of growth factor to PC12 cells causes them to differentiate into neuron-like cells that grow neurites and synthesize neurotransmitters.As useful as immortalized cell lines of neural origin can be for certain experiments, they show obviously abnormal traits, such as the unusual combination of neurotransmitters they produce (no normal neuron produces dopamine, norepinephrine, and acetylcholine in the same cell!). Immortalization has been achieved by SV40 but has resulted in cell lines with altered differentiation. An email will be sent to you with instructions. By continuing you agree to the Copyright © 2020 Elsevier B.V. or its licensors or contributors.
A 2010 toxicogenomics study demonstrated that HepaRG is a more suited in vitro liver model for biological interpretation of the effects of exposure to chemicals, whereas HepG2 is more promising for classification studies using the toxicogenomics approach Several immortalized cell lines and, as noted above, 10–15% of tumors are telomerase-negative and yet maintain functional telomeres. Most immortalised cell lines are classified by the cell type they originated from or are most similar to biologically. Our portfolio contains over a hundred unique cell line collections sourced from collaborations with life science research institutions and leading scientists. Immortalized cell lines accumulate mutations over time in culture and may lose the full spectrum of response that is found in the parental cells, again making assay validation essential.Immortal cell lines are an alternative to primary cultures.
Additionally, individual characteristic of the OS (e.g. Your account has been locked. Cell lines can be finite or continuous. The cell lines may mutate, and genetic drift or (usually unknown) selective pressures in culture may promote significant changes in phenotype; for example, PC12 cells from different laboratories may show different reponses compared to the original line (There are many advantages to using immortalized cell lines.
There are various immortal cell lines. However, most neuronal immortalized cell culture models are derived from tumors and are sometimes genomically abnormal. derived from stem cells).
Some viruses do not grow well in cultured cells, but may be coaxed to replicate in primary cells, embryonated eggs, or experimental animals. One popular neuronal cell line, called PC12, is a rat pheochromocytoma cell line derived from an adrenal gland tumor.
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